How to choose the right cell culture plate? -Huaqiang Electronic Network

Cell Culture Plates Cell culture plates are essential tools in biological and medical research. Depending on the shape of the bottom, they can be classified into flat-bottomed and round-bottomed (U-shaped or V-shaped). The number of wells varies, including 6, 12, 24, 48, 96, 384, etc. Materials also differ, such as Terasaki plates and standard cell culture plates. Choosing the right type depends on the type of cells being cultured, the required volume, and the experimental goals. Flat-bottomed plates are typically used for adherent cells, as they provide a stable surface for cell attachment. They are also ideal for microscopic observation due to their clear, flat base and uniform liquid level. These plates are commonly used for MTT assays, where cell viability is measured. On the other hand, round-bottomed plates (U-shaped or V-shaped) are often preferred for suspension cultures and experiments that require cell collection, such as isotope incorporation studies.

Cell Culture Plates Compared to microplates, cell culture plates are generally more cost-effective. While cell culture plates are primarily designed for cell growth, they can also be used for protein concentration measurements. In contrast, microplates are usually more specialized and expensive. They include coated plates and reaction plates, which are not suitable for direct cell culture. Instead, they are mainly used after immunoenzymatic reactions for protein detection, requiring specific enzyme solutions and higher technical standards.

The bottom area of different culture plates and the recommended liquid addition amount play a crucial role in maintaining optimal cell growth. The depth of the culture medium should not be too deep, ideally between 2-3 mm. This ensures proper gas exchange, especially oxygen, and prevents overflow during handling, which could lead to contamination. The appropriate volume for each well depends on the size of the well and the experiment’s purpose. Adjusting the cell density accordingly helps achieve better results in various experiments.

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