Kind
Product
Minute
Analysis
Newspaper
Report
Material: Hyper 0DS2 C18
Flow Rate: 1.0 ml/min
Mobile Phase: Acetonitrile: 0.1% Phosphate Water = 45:55
Pressure: 10.0 MPa
Column Length: 250 mm
Detector: DAD at 275 nm
Column Diameter: 4.6 mm
Injection Volume: 20 µL
#
Component Name
Retention Time (min)
Peak Height (mV)
Peak Area (mV·s)
Area Percentage (%)
1
Orange Cassia
10.10667 | 894.44 | 7872.97 | 99.7426
2. Unknown
11.02333 | 1.01 | 8.40 | 0.1064
3. Unknown
20.29167 | 0.59 | 11.91 | 0.1509
This report presents the detailed analysis of a sample of Orange Cassia, a natural compound known for its medicinal properties. The sample was identified as having the chemical formula Câ‚₇Hâ‚â‚„O₇ with a molecular weight of 330.28 g/mol. It is stored under controlled conditions at 4°C, in a sealed container, and away from direct light to preserve its integrity. The analysis was conducted using high-performance liquid chromatography (HPLC) on a Hyper 0DS2 C18 column, which is commonly used for separating complex organic compounds. The mobile phase consisted of acetonitrile and 0.1% phosphate buffer in a 45:55 ratio. The flow rate was maintained at 1.0 mL/min, and the column temperature was not specified but typically remains constant during such analyses. The detector used was a Diode Array Detector (DAD) set at 275 nm, a common wavelength for detecting flavonoids and related compounds. The injection volume was 20 µL, and the system pressure was recorded at 10.0 MPa, indicating normal operating conditions. The HPLC results show that the sample has a purity of 99.7%, which is well above the standard of 98%. The retention time for the main component, Orange Cassia, was approximately 10.11 minutes, with a peak height of 894.44 mV and a peak area of 7872.97 mV·s. This indicates a strong and consistent signal for the compound of interest. In addition to the main component, two unknown peaks were observed. The second peak had a retention time of 11.02 minutes, a small peak height of 1.01 mV, and a very low area percentage of 0.1064%. The third peak was detected at 20.29 minutes, with a peak height of 0.59 mV and an area percentage of 0.1509%. These minor components may represent impurities or other naturally occurring compounds present in the sample. Overall, the analysis confirms that the sample meets the required standards for purity and quality, making it suitable for further use in research or pharmaceutical applications.Photovoltaic Installation Tools
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